Introduction
Sizing a biofilter can be a daunting task for the novice in the aquaculture industry. There are many technologies and options to choose from. Once you have chosen a type of biofilter, there are still a tremendous number of variables to consider and make decisions about. Due to the wide disparity in the data reported in the literature, it is wise to oversize biofilters or provide for a modular design that will allow for expansion.
General Design Concerns
One of the most important considerations in any biofilter design is providing all the bacteria in the biofilm with a constant supply of food (nutrients) and oxygen. The reason the word “all” is emphasized is because many biofilter designs do not distribute the flow of food and oxygen evenly. Some parts of the biofilter may have a high flow of water past them and other parts may have almost no flow. This is a common cause of poor performance by a biofilter.
Even hydraulic loading (water flow) throughout the filter is extremely critical to proper utilization of the biofilter media. Rotating Biological Contactors (RBCs) are consistently shown to have very high treatment efficiencies. This is because the media is consistently and evenly moved through the water to be treated. All the bacteria on the surface area contained in the RBC are exposed to a steady stream of food and oxygen. Trickling filters and submerged filters can achieve high treatment efficiencies if they also have a constant, even flow of water to all the surface area contained in the filter.
Trickling Filters
This discussion begins with the design of trickling filters because they are easier to design and size than a submerged filter. Once the size and shape of the trickling filter has been decided, the water distribution system must be designed. It cannot be over emphasized that the water must be evenly distributed over the top of the media. Areas that are dry will not provide any biofiltration benefit. In addition, the interfacial area between the wet and dry areas will tend to accumulate solids and eventually lead to plugging. In general, it is easier to evenly distribute the water for systems with high hydraulic loadings compared to low hydraulic loadings. There is no maximum water loading for structured packing.
There are two common ways to get an even distribution using a fixed spray system. For square or rectangular vessels one way is to use solid cone, square pattern spray nozzles. The edge of each spray pattern should touch the edge of each adjacent pattern. This system is easy to visualize and design. The only requirement is that the nozzle spray pattern must be well defined by the manufacturer so that the designer can size the system properly. The most common drawback to this system is that not all nozzles have an even spray pattern even though they are sold as solid cone nozzles. Very often the center of the spray pattern has a lower water loading than the edge of the pattern.
The second way to get an even water distribution is to use many over lapping spray patterns. Often, the individual nozzle spray pattern is hollow but the combination of many patterns covering the same spot gives even coverage. The main problem with this system is that the wall of the vessel will intercept a certain amount of water. Any water that hits the wall will generally not go through the filter media. In a very large vessel, this is not a problem because the ratio of the perimeter to the area is small. In small vessels this system will not work well.
There is a third method of distributing water that can be used in round vessels. Rotating arm distribution systems can give an even distribution, if they are sized properly. They also have a low head compared to some spray systems. The drawback to rotating arm systems is the wear and tear on moving parts and the required maintenance.
Part of the art of designing a trickling filter is to balance the competing requirements on the design.
- To keep the energy costs to a minimum, the pumping head for the filter should be as low as possible. The maximum plan area covered by the filter is determined by the minimum water loading.
- To minimize the floor space used by the filter, the filter should be as tall as possible. The practical limitations are the height of the building, the head limits on the pump, and the structural and stability considerations of the vessel.
- A taller filter will have a longer flow path for the water. This means a more complete treatment of the water with each pass.
- Taller filters will have higher specific water loadings. This means better flushing action, more turbulent water films, and higher ammonium removal rates.
Trickling filters for industrial applications are sometimes 30ft (9.1m) tall. This is not practical for aquaculture systems. In general, trickling filters for aquaculture are between 3-10ft (1-3m) tall.
Submerged Filters
Submerged filters are excellent choices for small systems because they are very versatile. They can be located in a separate tank or in the culture tank. They can be horizontal-flow, up-flow, or down-flow. They can be aerated or not. The most important consideration for the design is the even distribution of water to the packing. Unfortunately it is very common for submerged filters to be designed as large, flat, and thin sections of packing with water direction being up-flow or down-flow. There is typically no provision for distributing the water to all areas of the media. The length of the water path through the media is very short, and the resistance to flow is very low. This is a recipe for disaster. The water flow will short circuit though a small section of the media and the rest of the biofilter will become anaerobic.

Figure 1: Up-Flow or Down-Flow Submerged Filter with Poor Hydraulic Design
Ideally the flow path through a submerged filter should be as long as possible. A long thin raceway is the best. This type of biofilter is known as an LPPF (Long Path, Plug Flow) filter.1 Another possible alternative is the use of aeration to induce a circulating flow around a tank. The key to properly designed submerged biofilters is the velocity through the media. The goal should always be to provide a high enough velocity through the media so that the flow is turbulent and all of the media has an equal flow of water. Equal flows of water ensure a consistent supply of oxygen and nutrients to all of the bacteria on the surface of the media.
Design Examples
Simplified Trickling Filter Design Process
Trickling filters are relatively easy to design and build. Here is an example of a simplified procedure for a trickling filter that may help to start the design process.
- Estimate the maximum amount of feed you will be feeding your fish at any time in the crop cycle. This will determine the maximum load the biofilter will need to handle.
- Decide or determine the TAN (Total Ammonia Nitrogen) level that your fish will be able to tolerate with no ill effects. Based on the allowable ammonia concentration in the culture tank, determine the ammonia removal rate. Make a guess about the hydraulic loading rate that you will use. See Figure 2.
Figure 2: Ammonium Removal Rate at Different Hydraulic Loading Rates2
- NOTE: Use this graph with caution!! This graph gives approximate removal rates for warm, freshwater trickling filters with good water distribution and sufficient dissolved oxygen.
- Saltwater systems will have lower removal rates
- Cold water systems will have lower removal rates.
- New systems will have lower removal rates.
- Systems that have wide swings in ammonia concentration will have lower removal rates.
- Nitrifying bacteria are inhibited by light so filters exposed to bright lights will have lower results.
- Systems using various therapeutic agents like formaldehyde can have lower results.
- You may get better results, but you can also get worse results. It is much better to include a generous safety factor than have a system limited by the biofilter. Lower ammonia concentrations are always better than higher concentrations. To the best of this author’s knowledge, no fish ever died as a result of an oversized biofilter, but many have died from undersized ones.
- NOTE: Use this graph with caution!! This graph gives approximate removal rates for warm, freshwater trickling filters with good water distribution and sufficient dissolved oxygen.
- Decide on the amount of water that will flow through the biofilter. One way to look at water flow is to calculate how long it will take for all the water in the culture tank to pass through the filtration system. A very slow system might recirculate all the water in 12hrs. A very fast system might recirculate all the water in 5min. A good starting point for a lightly loaded system might be a 1hr turnover time. In other words, all the water in the tank is passed through the filter in (1) hour. For example, a 5000gal (19,000 L) tank would need a flow rate of 5000gal/hr = 83GPM (315.5 L/s).
- If you are adding oxygen with a system that is external to your culture tank, your recirculating flow rate will probably be determined by the oxygen requirements of the fish. If your oxygen addition is within your culture tank, then the solids removal and biofiltration will guide your flow rate.
- Based on the amount of feed to be used and the ammonia removal rate, calculate the total amount of surface area required for the biofilter packing.
- Decide on the density or SSA (Specific Surface Area) of the biofilter material. The two commonly used SSAs for structured media are 69ft2/ft3 and 48 ft2/ft3 (226m2/m3 and 157m2/m3).
- Divide the total amount of required surface area by the SSA of the filter material. This gives you the volume of biofilter media required.
- Decide on the shape of the biofilter.
- Determine the hydraulic loading rate. If the hydraulic loading rate is too low, either increase the water flow or make the trickling filter taller and narrower. The hydraulic loading rate influences the ammonia removal rate as shown in Figure 2. In addition, there is a recommended minimum water loading rate based on the specific surface area of the media. The more surface area there is in a given volume, the more water is required to fully wet all the surfaces.
The following table shows recommended water loading rates for different media specific surface area values:

Table 1: SSA vs Minimum Water Loadings NOTE: The area (ft2 or m2) in the water loading column refers to the plan area (top area) of the trickling filter. The area in the specific surface area column refers to the surface area contained within the packing or media.
Example Sizing Calculation

Table 2: Example Sizing Calculations
If we start with a stack of media 4ft W x 4ft L x 5ft H (1.2m W x 1.2m L x 1.5m H) we get 80ft3 (2.21m3). Using the initial 83gpm flow rate above, the hydraulic loading would be about 5.2GPM/ft2 (3.53 L/s-m2). This is higher than the minimum hydraulic loading rate of 5GPM/ft2 for this media (from Table 1 above), but lower than the 6GPM/ft2 initially estimate used when reading Figure 2. The easy thing to do is to increase the water flow rate slightly to 96GPM (6.1L/s), based on the estimated 6GPM/ft2 (4.07L/s-m2) loading over the 4ft W x 4ft L (1.2m W x 1.2m L) plan area.
The above procedure is a simplified, general guideline for the process of designing a trickling filter. Predicting the amount of packing required for a given application is not an exact science. If the filter is undersized for the application, the level of TAN at design conditions may be higher than desired. Generally, it is wiser to oversize a biofilter rather than undersize it. Fish will not die from an oversized biofilter.
Footnotes
1See White Paper WP001 for more details on this type of filter.
2See Reference 2 on the last page of this paper for the source of this data.
3NOTE: The area (ft2 or m2) in the water loading column refers to the plan area (top area) of the trickling filter. The area in the specific surface area column refers to the surface area contained within the packing or media.
References
- Greiner, A. D., Timmons, M. B., 1998. Evaluation of the Nitrification Rates of Microbead and Trickling Filters in an Intensive Recirculating Tilapia Production Facility. Aquacultural Engineering pp.189-200.
- Kamstra, A., Van der Heul, J.W., Nijhof, M., 1998. Performance and Optimization of Trickling Filters on Eel Farms. Aquacultural Engineering pp.175-192.
- Saucier, B., Chen, S., Zhu, S., “Nitrification Potential and Oxygen Limitation in Biofilters” presented at the Third International Conference on Recirculating Aquaculture July 2000.
- Timmons, M.B., Losordo, T. M., 1994. Aquaculture Water Reuse Systems: Engineering Design and Management. Elsevier Science B.V.
- Zhu and Chen, 1999. An Experimental Study on Nitrification Biofilms Performances Using a Series Reactor System. Aquacultural Engineering, Vol 23, pp.245-259.
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Author: Matt Smith
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rev. 1/02/2025